T vector 和 T easy vector 的区别Thank you!

T vector 和 T easy vector 的区别Thank you! Easy-T-vector 与蓝白筛选我用买来的Easy-T-vector直接转化DH5a,在IPTG-X-gal平板上只有白色菌落,无蓝色菌落.这个现象正常吗?是不是因为这个载体因末端多出T,无法自连为环状,所以LacZ基因中断无法正 感受态细胞涂平板 什么菌落都没有长出来 做克隆实验,目的基因与pGEM-T Easy Vector连接后,和JM109感受态细胞培养,然后培养液在LB+氨卞青霉素+IPTG+X-Gal培养基上37度培养,结果没有长出来任何菌落. It wasn't easy waking up in a cell.But it would've been easier to quit 关于t载体基因工程中常提到t载体（t-Vector),请问什么是t载体,t载体有什么作用 十万火急～.1) A particle initially located at the origin has an acceleration of = 2.00 m/s2 and an initial velocity of 0 = 7.00 m/s.(a) Find the vector position at any time t (where t is measured in seconds).(____t + ____t2 ) mFind the vector ve promise don t come easy. What isn't easy about learning English和what isn't easy for learning English有什么区别 They've They haven’t don‘t it easy意思是不是等于take it easy? Promises Don't Come Easy 歌词 Love Don't Come Easy 歌词 these questions aren't easy _____(answer) It Ain't About Easy 歌词 Don't be nervous,please和Please take it easy是同义句吗? Don,t be nervious 和Take it easy有什么区别 Take it easy 和Don't be nervous.有什么不同 Take it easy.Don't be _______.后面写什么?stressed 和 stressed out 选哪一个